Figure 2: Surgical procedure and perioperative clinical and neurophysiological evaluation. Note: (A) Surgical procedure: starting from trigeminal nerve exposure (A1, 2); then fEMG evaluation (A3); followed by facial nerve combing (A4). (B) fEMG was used to evaluate amplitude reduction; the facial nerve was combed several times along the nerve fibers until the amplitude of the fEMG decreased by 30% to 50%. (C) The amplitudes of the fEMG traces decreased 1 week after operation by 31% to 75% in the patient with the least number of nerve combing events (7 times; case 1) and in the patient with the most number of nerve combing events (21 times; case 2). (D) There was a positive correlation between the number of combing events and intraoperative fEMG amplitude reduction rate (r = 0.5167, P = 0.004). The number of combing events is represented by different colors. (E) There was a positive correlation between the number of nerve combing events and HB grade at 7 days postoperation. (F) There was a negative correlation between the number of nerve combing events and the BSDI score at 7 days postoperation. A lower BSDI score means a better outcome. AICA: Anterior inferior cerebellar artery; BSDI: blepharospasm disability index; fEMG: free electromyography; G: Gelfoam; HB: House–Brackmann; IX: glossopharyngeal nerve; NSC: non-stem cell; post-op: postoperation; pre-op: preoperation; PV: petrosal vein; SCA: superior cerebellar artery; V: trigeminal nerve; VII: facial nerve; VIII: vestibular nerve.